by Jackie Willis - Entertainment Tonight

Amy Schumer decided to read an open letter during her stand-up show at New York City's Madison Square Garden on Tuesday night that called out the Trump supporters who walked out of her show in Tampa, Florida.

On Sunday night in the Sunshine State, the Trainwreck star asked a Trump supporter to come onstage and explain why he was voting for the presidential hopeful. She also referred to Trump as an "orange, sexual-assaulting, fake-college-starting monster."

"Dearest Tampa, I'm sorry you didn't want me, a comedian who talks about what she believes in, to mention the biggest thing going on in our country right now," Schumer read aloud to a cheering audience in NYC. "How could I think it was OK to spend five minutes having a peaceful conversation with someone with different views?"

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The 35-year-old comedian added, "After the show, I want you to know that I will go straight to a rehab facility that will teach me how to make all people happy."

Prior to hitting the stage on Tuesday evening, Schumer assured those attending that she would deliver a "squeaky clean show," calling it the "#TampaApologyTour."

And so are these gals! @rachelfeinstein_ @marknormand and I are putting on a squeaky clean show #msg #tampaapologytour

A photo posted by @amyschumer on

Schumer's open letter wasn't the only new addition to her stand-up show. Madonna also surprised the crowd when she opened for her comedian friend. "So much fun opening up for @amyschumer at Madison Square Garden tonight!!" the 58-year-old singer Instagrammed. "It was short but it was sweet (like me). I'm forever changed! Love you Amy!!"

Surprise! Madonna doing stand up comedy before Amy Schumer at the Madison Square Garden. πŸ‘ΈπŸΌπŸ˜±πŸ™ŒπŸ» pic.twitter.com/njWjWnMGCW

— Madonna Lately (@MadonnaDailyID) October 19, 2016

So much fun opening up for @amyschumer at Madison Square Garden tonight!! 🍾🍾🍾🍾. It was short but it was sweet (like me). I'm forever changed! πŸŽ‰πŸŽ‰πŸŽ‰πŸŽ‰πŸŽ‰ Love you Amy!! πŸ’˜πŸ’˜πŸ’˜

A photo posted by Madonna (@madonna) on

As fans may recall, Schumer opened for Madonna during her NYC shows in September 2015.

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"Love and Respect this woman so much!!" Madonna posted on Tuesday night. "Thanks for letting me get on your stage! It was AWESOME!! @amyschumer"

Love and Respect this woman so much!! Thanks for letting me get on your stage! It was AWESOME!! @amyschumer πŸŽ‰πŸŽ‰πŸŽ‰πŸ™πŸ»πŸ’˜πŸ˜‚πŸΎπŸΎ you were AWESOME!

A photo posted by Madonna (@madonna) on

Schumer also got the music icon to do a human pyramid with her pals, and shared the moment on Instagram.

Human pyramid starring @madonna

A photo posted by @amyschumer on

The Tampa show wouldn't be the first time Schumer has butted heads with some of her audience members. During her performance in Stockholm, Sweden over the summer, the comedian had a guy booted out of her show after he heckled her by yelling, "Show me your t*ts!"

Check out Schumer's response: